Biological Safety Manual

BIOLOGICAL SAFETY MANUAL

THE UNIVERSITY OF NORTH CAROLINA at CHAPEL HILL
Health and Safety Office
August 2000

To request a paper copy of the complete manual, please call EHS at 962-5507.

TABLE OF CONTENTS

Chapter 1	Introduction
Chapter 2	Occupational Hazards in Microbiological Laboratories
Chapter 3	Principles of Biosafety
Chapter 4	Description of Laboratory Biosafety Levels
Chapter 5	Description of Vertebrate Animal Biosafety Levels
Chapter 6	Risk Assessment
Chapter 7	Recommended Biosafety Levels for Infectious Agents and Infected Animals
Chapter 8	Agent Summary Statements Bacterial Agents Fungal Agents Parasitic Agents Prions Rickettsial Agents Viral Agents (other than arboviruses) Arboviruses and Related Zoonotic Viruses
Chapter 9	Biosafety in Laboratories Handling Human Blood
Chapter 10	Research Involving Recombinant DNA Molecules
Chapter 11	Hazards of Cell and Tissue Culture Systems
Chapter 12	Safe Handling of Laboratory Animals
Chapter 13	Laboratory Techniques for Biohazard Control
Chapter 14	Biological Safety Cabinets
Chapter 15	Decontamination and Disposal
Chapter 16	Clean Up of Biohazardous Spills
Chapter 17	Transportation and Transfer of Biological Agents
Chapter 18	Guidelines for Work With Toxins of Biological Origin
Chapter 19	Laboratory Security and Emergency Response Planning
App 15A	Decontamination and Disposal of Infectious Waste
Table 1	Comparison of Biological Safety Cabinet Characteristics and Applications

Environment, Health & Safety
1120 Estes Drive Extension, CB# 1650
Chapel Hill, NC 27599
Phone 919-962-5507 # Fax 919-962-0227