Chapter 2: Laboratory Safety Plan
This chapter provides detailed instructions for completing and updating the various schedules of a Laboratory Safety Plan. The Laboratory Safety Plan is a required document that outlines specific conditions, hazards, and controls in your laboratory spaces.
This chapter describes the required information to include in an LSP. The Laboratory Safety Plan web application allows a Principal Investigator to submit his or her LSP online, directly into the EHS database, rather than printing out a copy and mailing it to EHS.
The link to “Create a New Plan” is selected by new Principal Investigators and any PI that has previously submitted a paper copy of a Laboratory Safety Plan.
Step 1: Building Selection
A dropdown menu of all University buildings is provided. Remember, each building in which a PI has lab space will have its own Laboratory Safety Plan.
Step 2: Room Selection
A dropdown menu of rooms in that building is provided. Select rooms from this list or type in the space provided. Once all rooms are added, continue to Step 3.
Step 3: Hazard Selection
In the table, identify under the room number which schedules or type of operation occur in that room.
- Schedule B: Hazardous Chemicals and Equipment
- Schedule C: Radioactive Materials
- Schedule D: X-Ray Equipment
- Schedule E: Laser Equipment
- Schedule F: Biological Hazards
- Schedule G: Recombinant or Synthetic Nucleic Acid Molecules
- Schedule H: Transgenic Animals or Plants
- Schedule I: Shipping
The after-hours number can be a home phone number, cell phone number, or pager. First responders (EHS, UNC Public Safety, and Chapel Hill Fire Department) will use it to contact the PI or the Safety Supervisor in the event of an emergency after hours or on weekends.
- Location and availability of the Chemical Hygiene Plan (Laboratory Safety Manual and Laboratory Safety Plan). These documents can be referenced online. Printed copies are not required except for the sections of the LSP that are printed and posted.
- Location and availability of known reference material (including safety data sheets) on the hazards, safe handling, storage, and disposal of hazardous chemicals.
- Permissible Exposure Limits (PELs) for OSHA regulated substances and recommended exposure limits for non-regulated substances.
- Physical hazards and health hazards of chemicals in the workplace.
- Signs and symptoms associated with exposures to hazardous chemicals used.
- Documentation that each employee has received training from EHS on the OSHA Laboratory Standard. This information can be accessed online.
- Documented annual review of the Laboratory Safety Plan for each person. This review is done through the online Laboratory Safety Plan.
An online Chemical Inventory System (CIS) is part of the online Laboratory Safety Plan. This system includes several of the most common chemicals used in the laboratory in a drop-down menu format. Instructions for completing the CIS are included in Appendix 2-A. Please consult the SDS or contact EHS if you have any questions about proper classification of chemicals before the online CIS is completed.
- Detailed procedures for handling toxic chemicals and “select carcinogens” (Chapter 7) that are used in the laboratory, including designation of the work area and entry restrictions
- Identification of materials and procedures that are to be restricted to laboratory hoods and biological safety cabinets
- Designation of areas where eating or drinking is allowed, if any (also indicate areas on the floor plan)
- Actions to prevent damage to equipment
- Actions to prevent loss of experiments in progress
- Actions to secure hazardous material in use at the time an emergency is sounded
- Chemical spill response procedures
- Conduct a walk-through survey of laboratory to identify hazards for which eye, face, and hand PPE are required.
- Identify specific work areas, materials or chemicals in the space provided under “Laboratory Operation”.
- In the space under “Hazard” describe the potential hazards for which PPE is required.
- Under “PPE Required” describe the specific PPE to be worn when performing that work activity.
The procedures described in the application, as modified by the Radiation Safety Officer and/or the Radiation Safety Committee, become the conditions under which they authorize a researcher and his/her personnel to use radiation sources. The Radiation Safety Officer must review any subsequent change in procedure regarding the use, storage or disposal of sources prior to instituting the change. Please read the UNC Radiation Safety Manual for more information.
- biological toxin
- human/primate source material (including well established cell lines)
- recombinant DNA
Then indicate which room(s) is (are) used for handling and/or storage.
Next, complete the series of prompts. Note that the prompts are specific for that category of material, examples include diseases/symptoms, medical surveillance, vaccination, handling practices and precautions, surface disinfection, and waste treatment. If you do not locate the organism or material that you need to register, you will need to call EHS at 919-966-5507 to request the addition of that material.
Work at or above BSL-2 requires completion of the BSL-2 checklist. BSL-2 checklist items are derived from federal, state, and local regulations and requirements. Each room designated at BSL-2 must be evaluated against these criteria. Multiple rooms may be listed on a single form.
- Forms are due to Environment Health and Safety (CB #1650) by the 15th of the month.
- The Schedule G is required when you are making rDNA. The top portion of the document is for administrative information including Principal Investigator name, PID, CB number, etc.
- As a part of Schedule G you are required to classify your research using the link to the Classification Summary Page for NIH Guidelines.
- A project title is required. You will need to identify the proteins being produced under #2.
- If you click “yes” under #3, you must fill out Section III Entitled “Gene Transfer Experiments”.
- Number 4 asks you to specify your containment level. Please remember if your recipient organism is human cells lines then you must classify your project as BSL2. If the vector you are using (i.e. lentiviral vector) is a BSL2 vector then you will classify your containment level as BSL2.
- If you are working at BSL3 then your containment level is going to be BSL3.
- Please do not forget to describe the experiment fully under #8.
- When using vectors under Section III please remember to attach a map of the vector.
- In order for your Schedule G to be considered for approval, the entire form must be filled out and signed.
- Forms are due to Environment Health and Safety (CB #1650) by the 15th of the month.
- Schedule H is required to be filled out when you are acquiring transgenic mice from other researchers (including UNC) or outside companies such as Jackson labs. If your IACUC protocol lists transgenic mice, you will be required to provide the IACUC with an approved Schedule H. The Institutional Biosafety Committee must approve the Schedule H and send it to the IACUC.
- The top portion of the document is for administrative information including Principal Investigator name, PID, CB number, etc.
- A project title is required. Under #1 list where the animal was produced.
- It is important to fill out the entire form.
- Under containment level most Schedule H applications fall under BSL1 containment. If your animal is expressing human genes or is being transfected by replication competent vectors such as Adenovirus, Retroviruses or wild type Cowpox, then your animals would be classified as BSL2.
For returning users, log onto the EHS chemical inventory site with your Onyen and password. Select an option in the left hand menu to get started.
- Enter the Chemical Abstracts Service (CAS) number or chemical name into the “Chemical Search” box. Your chemical will show up in the drop down box. EHS has a database of over 60,000 chemicals, however if you do not find a match try an alternate name or a different spelling. Some chemicals in the database might not be listed under the name that you are more familiar with. Use the “User-Defined Name” to enter that information.
- Enter the “Quantity on Hand” and the unit of measure. You can also enter the concentration/molarity. Enter the “Maximum Quantity on Hand” if you would like to provide that information as well.
- Enter the chemical state (solid, liquid, or gas).
- Select the check box to indicate if you would like to “Share this Chemical?” with others. This option is to indicate that you would like to make some, or all, of this chemical available to someone else if they may need it. This reduces the cost of disposing hazardous materials and saves other researchers the expense of purchasing chemicals. This information is kept on a secure server.
- Select the “Building” and “Room” where the chemical is located.
- Enter the location within the room (i.e. cabinet, refrigerator, shelf, etc.). You can also optionally enter where in the location (e.g. top shelf, left side, or which refrigerator, freezer, etc.) the chemical is located.
- Add any “Notes” that you or EHS may find helpful when reviewing your inventory.
- Select compatibility groups (Chapter 4) associated with your chemical.
- Click the “Add Inventory Item” Button, and repeat for each chemical that you would like to add.
- Select the “Sort by” type and location you would like to view and click the [Search] button.
- Enter the new quantity and click the [Update] button to the right.
- If you no longer have the item, click the [Remove] button next to that inventory item.
- Click the chemical name if you would like to update any other information for that chemical.
Search Your Inventory
- Select the “Search by” type.
- Enter the information for the chemical you would like to find in your inventory. You can enter a partial term to find many chemicals. For example, if you type “a”, your search will return all the chemicals in your inventory that begin with the letter “a”.
- Click the [Search] button.
- Click the chemical name link for the one you would like to view/update.
Add New Chemical
If you would like to add a new chemical to the EHS database, please fill out the form and submit to EHS.
Inventory by Location
Create an inventory report where the results are listed by the location of the chemical. You can select all locations or just a specific one. Reports can be sorted by chemical name, CAS no., or date of inventory update.
Inventory by Building
Create an inventory report where the results are listed by the building(s) on your Laboratory Safety Plan. You can only select one building at a time, and then you can select all storage locations or just a specific one. Reports can be sorted by chemical name, CAS no., or date of inventory update.
Inventory with Compatibility Groups
Create a report of chemicals in your inventory and their associated compatibility group. Use this report to verify that incompatible chemicals are not being stored together and that storage areas are clearly labeled.