Self Study Unit:
Autoclave

• Introduction
  • Introduction
  • Proper Disposal of Biohazard Waste
  • Principles of Autoclave Operation
  • Gravity Displacement
  • Vacuum/ Gravity
  • Types of Autoclaves
  • Types of Autoclaves, cont.
  • Types of Autoclaves, cont.
• Before Autoclaving
  • Preventative Maintenance
  • Preventative Maintenance, cont.
  • Preventative Maintenance, cont.
  • Preventative Maintenance, cont.
  • Pre- Autoclaving Procedures
  • Pre- Autoclaving Procedures, cont.
• Proper Autoclave Use
  • What Can be Autoclaved
  • Proper Autoclave Use
  • PPE for Autoclave Users
• Hazards Associated With Autoclaves
  • Hazards
  • Hazards, cont.
  • Hazards, cont.
  • Hazards, cont.
  • Autoclave: Performance Indicators
• Autoclave Sterilization Procedures
  • Liquid Materials
  • Liquid Materials, cont.
  • Solid Materials
• Autoclave Procedures
  • Loading the Autoclave
  • Operating the Autoclave
  • Autoclave Explosion
  • Waste Decontamination Procedures
  • Waste Decontamination Procedures, cont.
  • Unloading the Autoclave
  • What's Wrong With These Pictures?
• Autoclave Testing and Verification
  • Testing and Verification
  • Testing and Verification, cont.
  • Testing and Verification, cont.
  • Testing and Verification, cont.
  • Testing and Verification, cont.
  • Testing Log
• Conclusion
  • Questions?

Principles of Autoclave Operation

Steam penetrates objects in the autoclave

Condensation creates negative pressure and draws in additional steam

Moist heat kills microorganisms via coagulation of proteins

Two types of autoclaves

1. Gravity Displacement
2. Vacuum/Gravity Assisted